A comparison was made of the soils of an area with absolute reserved regime and an area for grazing livestock in the Khomutovskaya Steppe Nature Reserve. According to the results of the analysis, livestock grazing significantly changes the granulometric composition of soils. The aggregate state of the soils in the humus-accumulative and illuvial horizons of the study areas is characterized by excellent condition according to the selected rating scale, but livestock grazing significantly aggravates this indicator.

The specific formation of the greenhouse plant collection of the Botanical Garden of the Institute of Biological Problems of the Cryolithozone of the Siberian Branch of the Russian Academy of Sciences is considered. The conditions of the growth of introduced plants in the specific natural and climatic conditions of Central Yakutia are highlighted. According to the results of the 2023 inventory, the collection includes 2,000 taxa, of which 500 species from 200 genera and 75 families. The largest number of genera and species diversity are represented by the families Araceae (11 genera, 45 species), Acanthaceae (9 genera, 12 species), Amaryllidaceae (9 genera, 10 species), Arecaceae (9 genera, 9 species), Crassulaceae (7 genera, 32 species), Bromeliaceae (7 genera, 8 species). In terms of life forms, the most numerous group consists of perennial grasses – 200 species. In terms of the number of species, the most widely represented are the Circumboreal (52 species), New Caledonian (41), Brazilian (32), Madagascar (32), Andean (26) and Mediterranean (12) floristic regions.

The intermediate results of the woody plants’ inventory of A.K. Malinovsky Botanical Garden of Ivanovo State University are presented. A total of 72 tree species from 33 genera, 15 families, 2 classes and 2 divisions were identified. The largest numbers of genera in the Magnoliophyta division belong to the Rosaceae family (7 genera), in the Pinophyta division the same relate to Pinaceae (5 genera). The richest genera in terms of species are Quercus L. (9 species, 13.8%) and Acer L. (7 species). Among the trees growing in the Botanical Garden, introduced species predominate – 58.3%, the share of native flora species is 41.7%. By geographical origin, the collection is dominated by species of the North American florogenetic element (18 species, 25%).

The history of cultivation of representatives of the genus Hemerocallis L. in Russia is considered. At present a significant reduction is noted in the distribution of retro varieties and their active replacement by modern varieties. At the same time, old varieties have an important set of features, the preservation of which in the cultural flora is an important task. Retro varieties of hybrid daylily remain promising in breeding work in order to obtain a stable regional assortment. Features of growth and flowering, vandal resistance and general unpretentiousness, simple agricultural technology and possibility of mass obtaining inexpensive planting material make the use of retro varieties in modern landscape design relevant.

The article presents a variant of the detailed classification of plant resources grouped according to their use, application, types of raw materials and biologically active substances. This classification comprises 13 groups and 66 subgroups with smaller divisions. Examples of plants are given for each classification unit. The role of plants in human life was studied basing on ethnobotanical data on the species available in the collection of the Sochi «Dendrarium». The collected gene pool forms the background for the development of many areas, including forestry with the production of valuable timber species, industries using natural raw materials, alternative energy, agriculture, food industry, forage production, biomaterial science, pharmaceutical industry, the creation of safe and comfortable human habitats in populated areas, the development of domestic tourism, education and raising environmental awareness.

The effect of different illumination levels on the germination of Psidium guajava L. seeds in the greenhouse complex of the Donetsk Botanical Garden was studied. It was determined that a 25 and 50 % decrease in the illumination level reduces the germination of P. guajava seeds by 22.5 % and 42.5 %, whereas the light level of 7124.3 ± 242.7 lux (without shading) facilitates germination of this species in 6,7 days earlier compared to the conditions when it was reduced by 25 % and 50 %. The statistical significance of the illumination level effect on the germination rates in P. guajava seeds was revealed.

The collection of economic valuable plants of the family Lamiaceae of the Donetsk Botanical Garden contains 58 species of aromatic medicinal plants, including 5 official and 28 species indicated as pharmacopoeial. Among the forage and medicinal plants used in veterinary medicine, there are 5 pharmacopoeial species. Herbaceous polycarpics, promising for cultivation in the open ground predominate in the collections. Medical, biological and phytochemical studies of some species, namely Agastache foeniculum (Pursh.) Kuntze, Ziziphora bungeana Lam., Thymus serpillum L. have shown the prospects for their use as potential sources of herbal medicines. The phytoresource potential of the collection of economic valuable plants of the Donetsk Botanical Garden has broad scientific and practical importance in terms of biodiversity conservation and enriching the region with useful plants of the world flora.

The current state of the collection fund of living plants in the Donetsk Botanical Garden and their bioresource significance, the fundamental scientific research areas on adaptation and acclimatization of world flora plants in the conditions of the steppe zone of Russia were analyzed. The directions are given in the research result application and use of collections in educational and environmental education activities, being the most important modern trend in the work of botanical gardens.

Some indicators of phytocenoses were investigated in the artificial steppe laid out 50 years ago in the area of the Donetsk Botanical Garden in comparison to those of different variants of natural steppe areas (petrophytic, typical and cretaceous) of Donbass. A comparative analysis has shown significant differences between the artificial steppe and natural areas in terms of species diversity and species saturation. Floristic diversity of the artificial steppe site is less compared to natural sites, but its species saturation is on the contrary greater. This fact is associated with the man-induced nature of vegetation formation in this area, that confirms its functional purpose – to be a pool for the conservation and reproduction of steppe plant species.

Two autochthonous species such as Noccaea macrantha (Lipsky) F. K. Mey, Phragmites tzvelevii Val. N. Tikhom. and one adventitious species, namely Tinantia erecta (Jacq.) Schetdl. are given for the first time for the flora of Donbass. The original texts of herbarium labels are presented and comments on them are cited.