As a result of the studies of plant-parasitic nematodes of trees and shrubs in the territory of Donetsk Botanical
Garden two species of Rotylenchus Filipjev, 1936 (Tylenchida: Hoplolaimidae) were recorded: R. buxophilus
Golden, 1956 was registered for the first time for Donbass and Ukraine, R. fallorobustus Sher, 1965 – for the
first time for Donbass and Left-bank Ukraine. Both species are polyphagous root semi-endoparasites.
Rotylenchus buxophilus was registered in 2020 in rhizosphere of common privet (Ligustrum vulgare L.), and
R. fallorobustus – in 2019 in rhizosphere of larch (Larix sp.). The paper contains descriptions, morphological
and morphometric characteristics of recorded populations, trophical associations, and brief data on the distri-
bution, biology, ecology and harmfulness of the species. The morphometrics of the Donbass populations of
both species generally are in agreement with literature data, but regional isolates have some differences.
A.I. Gubin