As a result of the studies of plant-parasitic nematodes of trees and shrubs on the territory of Donetsk Botanical Garden were recorded two species of Longidorus Micoletzky, 1922 (Dorylaimida: Longidoridae): L. elongatus (de Man, 1876) has previously been recorded in greenhouses of Donetsk Botanical Garden, L. leptocephalus Hooper, 1961 was registered for the first time for Donbass. Both species are polyphagous root ectoparasites. Longidorus elongatus was registered in rhizosphere of goat willow (Salix caprea L.), and L. leptocephalus — in rhizosphere of horse chestnut (Aesculus hippocastanum L.). The paper contains descriptions, morphological and morphometric characteristics of recorded populations, trophical associations, and brief data on the distribution, biology, eco-logy and harmfulness of the species. The morphometrics of the Donbass populations of both species generally are in agreement with literature data, but regional isolates has some differences.