The optimal cultivation period of the Trametes hirsute Th-11 strain was established, preliminary optimization of media with lignin-containing wastes composition was carried out with a view to increase the destruction efficiency. The vegetation experiment showed a significant effect of Trametes hirsute Th-11 fungus cultivated on media with lignin-containing wastes culture filtrate, and sunflower seed husk converted by Pleurotus ostreatus NK-35 on the aboveground and underground parts growth, lipid peroxidation intensity, peroxidase activity of Calendula officinalis L. seedlings under fluoride and sulрhite soil contamination conditions. The growth-stimulating and stress-protective properties of some lignin-containing wastes products transformed by fungi and enriched with fungal biologically active metabolites modifications were found. The study showed no unified pattern of the investigated substance influence on the physiological and biochemical parameters of Calendula officinalis seedlings under study.