Dendrotrophic powdery mildew fungi (Erysiphaceae) in urban plantings of Donetsk city agglomeration are presented by 37 fungal species (from which 13 species are first recorded) on 78 species of trees and shrubs. The most numerous genus Erysiphe R. Hedw. ex DC. is represented by 23 species, while the remaining 4 genera ( Arthrocladiella Vassilkov, Podosphaera Kunze, Phyllactinia Lé v. and Sawadaea Miyabe) — by 14 species only. There is a predominance of alien species (24 species, 64.9%), which can be divided into two main groups: true alien and relative alien species. Strong similarity in the species composition of dendrotrophic powdery mildew fungi of Donetsk city agglomeration and the analogical closest city agglomeration (Rostov-on-Don) is revealed, that could be interpreted as evidence of the commonality of the species composition formation, including the synchronism of the invasions of the Erysiphaceae alien species in the steppe zone of southeast Ukraine and the southern European Russia.